Semi-rational design of L-amino acid deaminase for production of pyruvate and D-alanine by Escherichia coli whole-cell biocatalyst.

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第一作者： Ke,Liu

第一单位： Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.

作者： Ke,Liu ^[1] ; Haoran,Yu ^[2] ; Guoyun,Sun ^[3] ; Yanfeng,Liu ^[2] ; Jianghua,Li ^[4] ; Guocheng,Du ^[1] ; Xueqin,Lv ^[3] ; Long,Liu ^[1]

作者单位： Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China. ^[1] College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China. ^[2] Science Center for Future Foods, Jiangnan University, Wuxi, 214122, China. ^[3] Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 311200, China. ^[4] Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China. lvxueqin@jiangnan.edu.cn. ^[5] Science Center for Future Foods, Jiangnan University, Wuxi, 214122, China. lvxueqin@jiangnan.edu.cn. ^[6] Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China. longliu@jiangnan.edu.cn. ^[7] Science Center for Future Foods, Jiangnan University, Wuxi, 214122, China. longliu@jiangnan.edu.cn. ^[8]

关键词 Genome engineering High-throughput screening L-amino acid deaminase Pyruvate Semi-rational design Whole-cell biocatalysis