Is amplification bias consequential in transposon sequencing (TnSeq) assays? A case study with a <i>Staphylococcus aureus</i> TnSeq library subjected to PCR-based and amplification-free enrichment methods.

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第一作者： Duah,Alkam

第一单位： Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.

作者： Duah,Alkam ^[1] ; Thidathip,Wongsurawat ^[2] ; Intawat,Nookaew ^[3] ; Anthony R,Richardson ^[3] ; David,Ussery ^[4] ; Mark S,Smeltzer ^[3] ; Piroon,Jenjaroenpun ^[5]

作者单位： Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA. ^[1] Division of Bioinformatics and Data Management for Research, Research Group and Research Network Division, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. ^[2] Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR, USA. ^[3] Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. ^[4] Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR, USA. ^[5]

关键词 Cas9 Nanopore PCR-bias PCR-free TnSeq nCATS transposon

主题词碱基序列(Base Sequence);DNA转座子(DNA Transposable Elements);基因文库(Gene Library);基因, 必要(Genes, Essential);基因组学(Genomics);高通量核苷酸序列分析(High-Throughput Nucleotide Sequencing);人类(Humans);诱变, 插入(Mutagenesis, Insertional);聚合酶链反应(Polymerase Chain Reaction);金黄色葡萄球菌(Staphylococcus aureus)

DOI 10.1099/mgen.0.000655

PMID 34596508

发布时间 2023-02-17

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Microbial genomics

2021年7卷10期

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Is amplification bias consequential in transposon sequencing (TnSeq) assays? A case study with a <i>Staphylococcus aureus</i> TnSeq library subjected to PCR-based and amplification-free enrichment methods.

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Is amplification bias consequential in transposon sequencing (TnSeq) assays? A case study with a <i>Staphylococcus aureus</i> TnSeq library subjected to PCR-based and amplification-free enrichment methods.

Microbial genomics

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Microbial genomics

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