Structural insights into the enhanced thermostability of cysteine substitution mutants of L-histidine decarboxylase from Photobacterium phosphoreum.
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关键词
Abbreviations: AADCs, amino acid decarboxylase; ASA, accessible surface area; CL, catalytic loop; HDC, L-histidine decarboxylase; HDH, histamine dehydrogenase; HsHDC, HDC from humans; LL, long loop; 1-methoxy PMS, 1-methoxy-5-methylphenazinium methylsulfate; MjTDC, tyrosine decarboxylase from Methanocaldococcus jannaschii; MR, molecular replacement; PLP, pyridoxal 5′-phosphate; PpHDC, L-histidine decarboxylase from Photobacterium phosphoreum; PpHDC-SM, PpHDC C57S mutant; PpHDC-TM, PpHDC C57S/C101V/C282V mutant; (WST)-8, water-soluble tetrazolium salt; WT, wild-typecrystal structurehistidine decarboxylaseprotein engineeringthermostabilitythermostable mutant
DOI
10.1093/jb/mvab103
PMID
34622278
发布时间
2022-03-31
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