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Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli.

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第一作者： Melanie R,Silvis

第一单位： Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA.

作者： Melanie R,Silvis ^[1] ; Manohary,Rajendram ^[2] ; Handuo,Shi ^[3] ; Hendrik,Osadnik ^[1] ; Andrew N,Gray ^[1] ; Spencer,Cesar ^[3] ; Jason M,Peters ^[4] ; Cameron C,Hearne ^[1] ; Parth,Kumar ^[1] ; Horia,Todor ^[1] ; Kerwyn Casey,Huang ^[5] ; Carol A,Gross ^[1]

作者单位： Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA. ^[1] Department of Bioengineering, Stanford Universitygrid.168010.e, Stanford, California, USA. ^[2] Department of Microbiology and Immunology, Stanford Universitygrid.168010.e School of Medicine, Stanford, California, USA. ^[3] Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin, USA. ^[4] Department of Bioengineering, Stanford Universitygrid.168010.e, Stanford, California, USA.;Department of Microbiology and Immunology, Stanford Universitygrid.168010.e School of Medicine, Stanford, California, USA.;Chan Zuckerberg Biohub, San Francisco, California, USA. ^[5]

关键词 CRISPRi Escherichia coli arrayed library essential genes microscopy

主题词细菌蛋白质类(Bacterial Proteins);大肠杆菌(Escherichia coli);基因敲低技术(Gene Knockdown Techniques);基因文库(Gene Library);基因, 必要(Genes, Essential);高通量筛选分析(High-Throughput Screening Assays);转录, 遗传(Transcription, Genetic)

DOI 10.1128/mBio.02561-21

PMID 34634934

发布时间 2024-04-03

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