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A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in Vero E6 cells.

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第一作者： Barbara,Storti

第一单位： NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12, 56127 Pisa, Italy.

作者： Barbara,Storti ^[1] ; Paola,Quaranta ^[2] ; Cristina,Di Primio ^[3] ; Nicola,Clementi ^[4] ; Nicasio,Mancini ^[4] ; Elena,Criscuolo ^[4] ; Pietro Giorgio,Spezia ^[2] ; Vittoria,Carnicelli ^[5] ; Giulia,Lottini ^[2] ; Emanuele,Paolini ^[6] ; Giulia,Freer ^[2] ; Michele,Lai ^[2] ; Mario,Costa ^[3] ; Fabio,Beltram ^[1] ; Alberto,Diaspro ^[7] ; Mauro,Pistello ^[8] ; Riccardo,Zucchi ^[5] ; Paolo,Bianchini ^[9] ; Giovanni,Signore ^[10] ; Ranieri,Bizzarri ^[11]

作者单位： NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12, 56127 Pisa, Italy. ^[1] Retrovirus Center, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, SS 12 Dell'Abetone e del Brennero 2, 56127 Pisa, Italy. ^[2] Istituto di Neuroscienze - CNR, Via Moruzzi 1, 56124 Pisa, Italy. ^[3] Laboratory of Medical Microbiology and Virology, University "Vita-Salute" San Raffaele, Via Olgettina, 58, 20132 Milan, Italy.;Laboratory of Medical Microbiology and Virology, IRCCS San Raffaele Hospital, Milan, Italy. ^[4] Department of Surgical, Medical and Molecular Pathology, and Critical Care Medicine, University of Pisa, Via Roma 65, 5616 Pisa, Italy. ^[5] Department of Mathematics, University of Pisa, Largo Bruno Pontecorvo 5, 56127 Pisa, Italy. ^[6] DIFILAB, Dipartimento di Fisica, Università degli Studi di Genova, Via Dodecaneso 33, 16146 Genova, Italy.;Nanoscopy, CHT, Istituto Italiano di Tecnologia, Via E. Melen 83, 16152 Genoa, Italy. ^[7] Retrovirus Center, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, SS 12 Dell'Abetone e del Brennero 2, 56127 Pisa, Italy.;Pisa University Hospital, Via Pietro Trivella, 56126, Pisa, Italy. ^[8] Nanoscopy, CHT, Istituto Italiano di Tecnologia, Via E. Melen 83, 16152 Genoa, Italy. ^[9] Fondazione Pisana per la Scienza, via F. Giovannini 13, 56017 San Giuliano Terme, PI, Italy. ^[10] NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12, 56127 Pisa, Italy.;Department of Surgical, Medical and Molecular Pathology, and Critical Care Medicine, University of Pisa, Via Roma 65, 5616 Pisa, Italy.;Nanoscopy, CHT, Istituto Italiano di Tecnologia, Via E. Melen 83, 16152 Genoa, Italy. ^[11]

关键词 ACE2, Angiotensin-Converting Enzyme 2 B.1.1.7 variant of concern Clathrin ISM, Image Scanning Microscopy Late entry SARS-CoV-2 spike SMLM, Single Molecule Localization Microscopy STED STED, STimulated Emission Depletion STORM, STochastic Optical Reconstruction Microscopy TIRF, Total Internal Reflection Fluorescence TMPRSS2, TransMembrane PRoteaSe Serine 2 dSTORM

DOI 10.1016/j.csbj.2021.10.038

PMID 34745450

发布时间 2021-11-25

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