Heterologous expression, characterization and evolution prediction of a diaphorase from Geobacillus sp. Y4.1MC1.

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作者： Jinzhao,Shang ^[1] ; Shuohao,Yue ^[2] ; Fang,Zeng ^[1] ; Yun,Chen ^[3] ; Longgang,Jia ^[4]

作者单位： College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. ^[1] College of Bioengineering and Food, Hubei University of Technology, Wuhan, 430068, China. ^[2] Department of Clinical Medicine, Fenyang College of Shanxi Medical University, Fenyang, 032200, China. ^[3] College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. longgangjia@tust.edu.cn. ^[4] College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. longgangjia@tust.edu.cn. ^[5]

关键词 Diaphorase Enzymatic properties Expression and purification Molecular dynamics simulation Mutant prediction

主题词克隆, 分子(Cloning, Molecular);酶稳定性(Enzyme Stability);大肠杆菌(Escherichia coli);土芽孢杆菌属(Geobacillus);氢离子浓度(Hydrogen-Ion Concentration);重组蛋白质类(Recombinant Proteins);温度(Temperature)

DOI 10.1007/s10529-021-03215-w

PMID 35001212

发布时间 2022-03-16

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