Cryopreservation of undifferentiated and differentiated human neuronal cells.

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作者： Kenji,Yamatoya ^[1] ; Yuya,Nagai ^[2] ; Naozumi,Teramoto ^[1] ; Woojin,Kang ^[3] ; Kenji,Miyado ^[4] ; Kazuya,Nakata ^[4] ; Tohru,Yagi ^[1] ; Yoshitaka,Miyamoto ^[5]

作者单位： Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan. ^[1] Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu, Chiba, 279-0021, Japan. ^[2] Department of Applied Chemistry, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba, 275-0016, Japan. ^[3] Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo, 157-8535, Japan. ^[4] Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo, 184-0012, Japan. ^[5] Department of Mechanical Engineering, Tokyo Institute of Technology, 12-2-1 Ookayama, Meguro-ku, Tokyo, 152-8552, Japan. ^[6]

关键词 5-CFDA-AM, 5-carboxyfluorescein diacetate BSA, bovine serum albumin Cryopreservation D-MEM, Dulbecco's Modified Eagle's Medium with high glucose, l-glutamine, phenol red, and sodium pyruvate D-PBS-free, Dulbecco's phosphate-buffered saline without Ca2+ and Mg2+ supplementation DAPI, 4′,6-Diamidino-2-phenylindole, dihydrochloride DMSO, dimethyl sulfoxide Differentiation EDTA, ethylene-diamine-tetraacetic acid ESC, embryonic stem cells FBS, fetal bovine serum NeuN, a neuronal marker Neuronal cells Regenerative therapy SDS, sodium dodecyl sulfate Sericin Serum-free iPSC, induced pluripotent stem cells

DOI 10.1016/j.reth.2021.12.007

PMID 35059480

发布时间 2022-01-22

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Regenerative therapy

2022年19卷

58-68页

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Cryopreservation of undifferentiated and differentiated human neuronal cells.

Regenerative therapy

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Cryopreservation of undifferentiated and differentiated human neuronal cells.

Regenerative therapy

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Regenerative therapy

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