Engineering of a UDP-Glycosyltransferase for the Efficient Whole-Cell Biosynthesis of Siamenoside I in <i>Escherichia coli</i>.

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作者： Yuncong,Xu ^[1] ; Shiqiang,Liu ^[1] ; Liuyun,Bian ^[1] ; Zhenlin,Li ^[2] ; Chen,Luo ^[1] ; Yijun,Chen ^[3] ; Xuri,Wu ^[1]

作者单位： Department of Biochemistry, College of Life Sciences and Technology, China Pharmaceutical University, 639 Longmian Road, Nanjing, Jiangsu Province 211198, PR China. ^[1] Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine, 100 Shizi Street. Hongshan Road, Nanjing, Jiangsu Province 210028, PR China. ^[2] Laboratory of Chemical Biology, College of Life Sciences and Technology, China Pharmaceutical University, 639 Longmian Road, Nanjing, Jiangsu Province 211198, PR China. ^[3]

关键词 UDP-glycosyltransferase enzyme engineering mogroside siamenoside I whole-cell biocatalyst

主题词葫芦科(Cucurbitaceae);大肠杆菌(Escherichia coli);葡糖苷类(Glucosides);糖基转移酶类(Glycosyltransferases);蛋白质工程(Protein Engineering);尿苷二磷酸(Uridine Diphosphate);尿苷二磷酸葡糖(Uridine Diphosphate Glucose)

DOI 10.1021/acs.jafc.1c07699

PMID 35099964

发布时间 2022-05-31

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