A warm-start digital CRISPR/Cas-based method for the quantitative detection of nucleic acids.

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作者： Xiaolin,Wu ^[1] ; Cheryl,Chan ^[1] ; Stacy L,Springs ^[2] ; Yie Hou,Lee ^[1] ; Timothy K,Lu ^[3] ; Hanry,Yu ^[4]

作者单位： Critical Analytics for Manufacturing Personalized Medicine Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, 138602, Singapore. ^[1] Critical Analytics for Manufacturing Personalized Medicine Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, 138602, Singapore; Center for Biomedical Innovation, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. ^[2] Critical Analytics for Manufacturing Personalized Medicine Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, 138602, Singapore; Synthetic Biology Center, Massachusetts Institute of Technology (MIT), Cambridge, MA, 02139, USA; Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology (MIT), Cambridge, MA, 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, MA, 02142, USA; Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA, 02142, USA. Electronic address: timlu@mit.edu. ^[3] Critical Analytics for Manufacturing Personalized Medicine Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, 138602, Singapore; Institute of Bioengineering and Bioimaging (IBB), A∗STAR, the Nanos, #04-01, 31 Biopolis Way, 138669, Singapore; Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, 117411, Singapore; Department of Physiology & the Institute for Digital Medicine (WisDM), Yong Loo Lin School of Medicine, MD9-04-11, 2 Medical Drive, 117593, Singapore. Electronic address: phsyuh@nus.edu.sg. ^[4]

关键词 CRISPR/Cas Isothermal amplification Nucleic acids detection and quantification Virus detection and quantification Warmstart digital CRISPR

主题词人类(Humans);核酸扩增技术(Nucleic Acid Amplification Techniques);核酸类(Nucleic Acids);RNA, 病毒(RNA, Viral);敏感性与特异性(Sensitivity and Specificity)

DOI 10.1016/j.aca.2022.339494

PMID 35151407

发布时间 2022-02-15

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准确一般字义偏差语意偏离译文作用小提交