Brick into the Gateway (BiG): A novel approach for faster cloning combining Golden Gate and Gateway methods.

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第一作者： Letícia F,Ferigolo

第一单位： Laboratory of Molecular Genetics of Plant Development, Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), University of São Paulo, 13418-900 Piracicaba, São Paulo, Brazil.

作者： Letícia F,Ferigolo ^[1] ; Mateus H,Vicente ^[1] ; Fabio T S,Nogueira ^[2]

作者单位： Laboratory of Molecular Genetics of Plant Development, Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), University of São Paulo, 13418-900 Piracicaba, São Paulo, Brazil. ^[1] Laboratory of Molecular Genetics of Plant Development, Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), University of São Paulo, 13418-900 Piracicaba, São Paulo, Brazil. Electronic address: ftsnogue@usp.br. ^[2]

关键词 Cloning DNA construct Gateway method Golden Gate GoldenBraid

主题词克隆, 分子(Cloning, Molecular);遗传载体(Genetic Vectors);质粒(Plasmids)

DOI 10.1016/j.plasmid.2022.102630

PMID 35398459

发布时间 2022-07-30

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