The structures of the C146A variant of the amidase from Pyrococcus horikoshii bound to glutaramide and acetamide suggest the basis of amide recognition.

导出 LinkOut

翻译打印收藏纠错

第一作者： Stanley,Makumire

第一单位： Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory 7925, South Africa. Electronic address: stanmakster@gmail.com.

作者： Stanley,Makumire ^[1] ; Siyun,Su ^[2] ; Brandon William,Weber ^[3] ; Jeremy David,Woodward ^[4] ; Serah,Wangari Kimani ^[5] ; Roger,Hunter ^[6] ; Bryan Trevor,Sewell ^[7]

作者单位： Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory 7925, South Africa. Electronic address: stanmakster@gmail.com. ^[1] Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory 7925, South Africa. Electronic address: siynsux@foxmail.com. ^[2] Electron Microscope Unit, Aaron Klug Centre for Imaging and Analysis, University of Cape Town, Rondebosch, Cape Town 7700, South Africa. ^[3] Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory 7925, South Africa; Electron Microscope Unit, Aaron Klug Centre for Imaging and Analysis, University of Cape Town, Rondebosch, Cape Town 7700, South Africa. Electronic address: jeremy.woodward@uct.ac.za. ^[4] Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory 7925, South Africa. ^[5] Department of Chemistry, University of Cape Town, South Africa. Electronic address: roger.hunter@uct.ac.za. ^[6] Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory 7925, South Africa. Electronic address: trevor.sewell@uct.ac.za. ^[7]

关键词 Amidase Amide substrate Hydrolysis Nitrilase Pyrococcus