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Continuous Percoll Gradient Centrifugation of Erythrocytes-Explanation of Cellular Bands and Compromised Age Separation.

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第一作者： Felix,Maurer

第一单位： Dynamics of Fluids, Experimental Physics, Saarland University, 66123 Saarbrücken, Germany.

作者： Felix,Maurer ^[1] ; Thomas,John ^[1] ; Asya,Makhro ^[2] ; Anna,Bogdanova ^[2] ; Giampaolo,Minetti ^[3] ; Christian,Wagner ^[4] ; Lars,Kaestner ^[5]

作者单位： Dynamics of Fluids, Experimental Physics, Saarland University, 66123 Saarbrücken, Germany. ^[1] Red Blood Cell Research Group, Institute of Veterinary Physiology, University of Zürich, CH-8057 Zürich, Switzerland. ^[2] Laboratories of Biochemistry, Department of Biology and Biotechnology "L Spallanzani", University of Pavia, I-27100 Pavia, Italy. ^[3] Dynamics of Fluids, Experimental Physics, Saarland University, 66123 Saarbrücken, Germany.;Physics and Materials Science Research Unit, University of Luxembourg, L-1511 Luxembourg, Luxembourg. ^[4] Dynamics of Fluids, Experimental Physics, Saarland University, 66123 Saarbrücken, Germany.;Theoretical Medicine and Biosciences, Medical Faculty, Saarland University, 66421 Homburg, Germany. ^[5]

关键词 Percoll age separation aggregation band 4.1 protein band formation blood sedimentation complex fluids density gradient micromechanical modeling red blood cells

医学主题词细胞分离(Cell Separation);离心法, 梯密度(Centrifugation, Density Gradient);红细胞(Erythrocytes);聚维酮(Povidone);二氧化硅(Silicon Dioxide)

DOI 10.3390/cells11081296

PMID 35455975

发布时间 2022-07-16

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