Chromatin Immunoprecipitation in Human Pluripotent Stem Cell-Derived 3D Organoids to Analyze DNA-Protein Interactions.

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作者： Wei Xuan,Tan ^[1] ; Chek Mei,Bok ^[2] ; Natasha Hui Jin,Ng ^[1] ; Adrian Kee Keong,Teo ^[1]

作者单位： Stem Cells and Diabetes Laboratory, Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore. ^[1] Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore. ^[2] Stem Cells and Diabetes Laboratory, Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore. ateo@imcb.a-star.edu.sg. ^[3] Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore. ateo@imcb.a-star.edu.sg. ^[4] Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore. ateo@imcb.a-star.edu.sg. ^[5]

关键词 3D Beta cell ChIP Chromatin immunoprecipitation Differentiation Islet Organoids Pluripotent Stem cell Transcription factor

主题词细胞分化(Cell Differentiation);染色质免疫沉淀法(Chromatin Immunoprecipitation);DNA(DNA);人类(Humans);类器官(Organoids);多潜能干细胞(Pluripotent Stem Cells)

DOI 10.1007/978-1-0716-1979-7_14

PMID 35507164

发布时间 2022-05-23

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