CRISPR-Cas12a combination to alleviate the false-positive in loop-mediated isothermal amplification-based diagnosis of Neisseria meningitidis.

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作者： Ngo Tat,Trung ^[1] ; Le Huu Phuc,Son ^[2] ; Trinh Xuan,Hien ^[3] ; Dao Thanh,Quyen ^[4] ; Mai Hong,Bang ^[5] ; Le Huu,Song ^[6]

作者单位： Centre for Genetics Consultation and Cancer Screening (CGC), Hanoi, Vietnam. tatrungngo@gmail.com. ^[1] Vietnamese-German Center for Medical Research (VG-CARE), Hanoi, Vietnam. tatrungngo@gmail.com. ^[2] Faculty of Tropical and Infectious Diseases, 108 Military Central Hospital, Hai Ba Trung District, No 1, Tran Hung Dao Street, Hanoi, Vietnam. tatrungngo@gmail.com. ^[3] 108 Institute of Clinical Medical and Pharmaceutical Sciences, Hanoi, Vietnam. tatrungngo@gmail.com. ^[4] Centre for Genetics Consultation and Cancer Screening (CGC), Hanoi, Vietnam. ^[5] Vietnamese-German Center for Medical Research (VG-CARE), Hanoi, Vietnam. ^[6] Faculty of Tropical and Infectious Diseases, 108 Military Central Hospital, Hai Ba Trung District, No 1, Tran Hung Dao Street, Hanoi, Vietnam. ^[7] 108 Institute of Clinical Medical and Pharmaceutical Sciences, Hanoi, Vietnam. ^[8] Vietnamese-German Center for Medical Research (VG-CARE), Hanoi, Vietnam. lehuusong@108-icid.com. ^[9] Faculty of Tropical and Infectious Diseases, 108 Military Central Hospital, Hai Ba Trung District, No 1, Tran Hung Dao Street, Hanoi, Vietnam. lehuusong@108-icid.com. ^[10] 108 Institute of Clinical Medical and Pharmaceutical Sciences, Hanoi, Vietnam. lehuusong@108-icid.com. ^[11]

关键词 CRISPR Cas12a LAMP Nesseria menitigistis PCR

主题词 DNA(DNA);人类(Humans);分子诊断技术(Molecular Diagnostic Techniques);脑膜炎奈瑟球菌(Neisseria meningitidis);核酸扩增技术(Nucleic Acid Amplification Techniques);RNA(RNA)

DOI 10.1186/s12879-022-07363-w

PMID 35508977

发布时间 2022-07-16

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