An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations.

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第一作者： Zhike,Lu

第一单位： College of Life Sciences, Zhejiang University, Hangzhou 310058, China.

作者： Zhike,Lu ^[1] ; Ke,Ni ^[2] ; Yingying,Wang ^[3] ; Yangfan,Zhou ^[2] ; Yini,Li ^[3] ; Jianfeng,Yan ^[4] ; Qingkai,Song ^[2] ; Min,Liu ^[3] ; Yujun,Xu ^[5] ; Zhenxing,Yu ^[1] ; Tiannan,Guo ^[2] ; Lijia,Ma ^[3]

作者单位： College of Life Sciences, Zhejiang University, Hangzhou 310058, China. ^[1] Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China. ^[2] Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024, China. ^[3] Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang 310024, China. ^[4] School of Life Sciences, Fudan University, Shanghai 200433, China. ^[5]

主题词 CD8阳性T淋巴细胞(CD8-Positive T-Lymphocytes);基因文库(Gene Library);淋巴细胞活化(Lymphocyte Activation);肿瘤(Neoplasms)

DOI 10.1093/nar/gkac458

PMID 35670669

发布时间 2024-01-04

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准确一般字义偏差语意偏离译文作用小提交