首页> Methods in molecular biology (Clifton, N.J.)> Solubilization and Purification of α5β1 Integrin from Rat Liver for Reconstitution into Nanodiscs.

Solubilization and Purification of α5β1 Integrin from Rat Liver for Reconstitution into Nanodiscs.

导出 LinkOut

翻译打印收藏纠错

第一作者： Estelle,Dransart

第一单位： Institut Curie, PSL Research University, Cellular and Chemical Biology unit, Endocytic Trafficking and Intracellular Delivery team, U1143 INSERM, UMR3666 CNRS, 26 rue d'Ulm, Paris Cedex 05, France.

作者： Estelle,Dransart ^[1] ; Aurélie,Di Cicco ^[2] ; Ahmed,El Marjou ^[3] ; Daniel,Lévy ^[2] ; Staffan,Johansson ^[4] ; Ludger,Johannes ^[5] ; Massiullah,Shafaq-Zadah ^[6]

作者单位： Institut Curie, PSL Research University, Cellular and Chemical Biology unit, Endocytic Trafficking and Intracellular Delivery team, U1143 INSERM, UMR3666 CNRS, 26 rue d'Ulm, Paris Cedex 05, France. ^[1] Institut Curie, PSL Research University, UMR 168, Centre de Recherche, Paris, France. ^[2] Recombinant Protein Facility, Institut Curie, Paris, France. ^[3] Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden. ^[4] Institut Curie, PSL Research University, Cellular and Chemical Biology unit, Endocytic Trafficking and Intracellular Delivery team, U1143 INSERM, UMR3666 CNRS, 26 rue d'Ulm, Paris Cedex 05, France. ludger.johannes@curie.fr. ^[5] Institut Curie, PSL Research University, Cellular and Chemical Biology unit, Endocytic Trafficking and Intracellular Delivery team, U1143 INSERM, UMR3666 CNRS, 26 rue d'Ulm, Paris Cedex 05, France. massiullah.shafaq-zadah@curie.fr. ^[6]

关键词 Electron microscopy FPLC FnIII9–10-functionalized affinity column Micelles Nanodiscs Negative staining Rat liver SDS-PAGE WGA-functionalized affinity column α5β1 integrin

主题词动物(Animals);细胞黏附(Cell Adhesion);细胞间通讯(Cell Communication);冷冻电子显微镜检查(Cryoelectron Microscopy);整合素类(Integrins);肝(Liver);大鼠(Rats)

DOI 10.1007/978-1-0716-2368-8_1

PMID 35773574

发布时间 2022-08-15

翻译满意度评价：

准确一般字义偏差语意偏离译文作用小提交