A joint PCR-based gene-targeting method using electroporation in the pathogenic fungus Trichosporon asahii.

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作者： Yasuhiko,Matsumoto ^[1] ; Tae,Nagamachi ^[2] ; Asami,Yoshikawa ^[2] ; Tsuyoshi,Yamada ^[3] ; Takashi,Sugita ^[4]

作者单位： Department of Microbiology, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo, 204-8588, Japan. ymatsumoto@my-pharm.ac.jp. ^[1] Department of Microbiology, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo, 204-8588, Japan. ^[2] Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo, 192-0395, Japan. ^[3] Asia International Institute of Infectious Disease Control, Teikyo University, 2-11-1, Kaga, Itabashi-ku, Tokyo, 173-8605, Japan. ^[4]

关键词 Electroporation Gene targeting Joint PCR Mutant Trichosporon asahii

DOI 10.1186/s13568-022-01431-9

PMID 35834071

发布时间 2022-07-18

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