Utilization of SUMO Tag and Freeze-thawing Method for a High-level Expression and Solubilization of Recombinant Human Angiotensinconverting Enzyme 2 (rhACE2) Protein in <i>E. coli</i>.

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作者： Mozafar,Mohammadi ^[1] ; Ramezan Ali,Taheri ^[2] ; Peyman,Bemani ^[3] ; Mohammad Sadegh,Hashemzadeh ^[2] ; Gholamreza,Farnoosh ^[1] ; Razieh,Amini ^[4]

作者单位： Applied Biotechnology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran. ^[1] Nanobiotechnology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran. ^[2] Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. ^[3] Department of Molecular Medicine and Genetics, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran. ^[4]

关键词 ACE2 RBD SARS-CoV-2 SUMO tag inclusion body mild solubilization rhACE2

主题词大肠杆菌(Escherichia coli);人类(Humans);蛋白质结合(Protein Binding);小泛素相关修饰蛋白质类(Small Ubiquitin-Related Modifier Proteins);尿素(Urea)

DOI 10.2174/0929866529666220715101357

PMID 35838231

发布时间 2022-09-28

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