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Comparative study of keratinocyte primary culture methods from paediatric skin biopsies for RNA-sequencing.

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第一作者： Déborah,Salik

第一单位： Department of Dermatology, CHU Saint-Pierre, CHU Brugmann and Hôpital Universitaire des Enfants Reine Fabiola, Université Libre de Bruxelles, Brussels, Belgium.

作者： Déborah,Salik ^[1] ; Yousra,El Kaderi ^[1] ; Christine,Hans ^[2] ; Anne,Lefort ^[3] ; Frederick,Libert ^[3] ; Guillaume,Smits ^[4]

作者单位： Department of Dermatology, CHU Saint-Pierre, CHU Brugmann and Hôpital Universitaire des Enfants Reine Fabiola, Université Libre de Bruxelles, Brussels, Belgium. ^[1] Cytogenetics Laboratory, Hôpital Erasme, ULB Center of Human Genetics, Université Libre de Bruxelles (ULB), Brussels, Belgium. ^[2] I.R.I.B.H.M, Campus Erasme, Université Libre de Bruxelles, Brussels, Belgium.;Brussels Interuniversity Genomics High Throughput core (BRIGHTcore), Campus Erasme, Université Libre de Bruxelles, Brussels, Belgium. ^[3] Department of Genetics, Hôpital Erasme, ULB Center of Human Genetics, Université Libre de Bruxelles (ULB), Brussels, Belgium.;Department of Genetics, Hôpital Universitaire des Enfants Reine Fabiola, ULB Center of Human Genetics, Université Libre de Bruxelles (ULB), Brussels, Belgium.;Interuniversity Institute of Bioinformatics in Brussels, Université Libre de Bruxelles, Brussels, Belgium. ^[4]

关键词 RNA-sequencing direct explant method enzymatic method keratinocyte primary cell culture