High-definition electroporation: Precise and efficient transfection on a microelectrode array.

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作者： Bastien,Duckert ^[1] ; Maarten,Fauvart ^[2] ; Peter,Goos ^[3] ; Tim,Stakenborg ^[4] ; Liesbet,Lagae ^[5] ; Dries,Braeken ^[6]

作者单位： Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200d, 3001, Leuven, Belgium; imec, Kapeldreef 75, 3001, Leuven, Belgium. Electronic address: bastien.duckert@imec.be. ^[1] imec, Kapeldreef 75, 3001, Leuven, Belgium. Electronic address: maarten.fauvart@imec.be. ^[2] Department of Biosystems, KU Leuven, Willem de Croylaan 42, 3001, Leuven, Belgium; Department of Engineering Management, University of Antwerp, Prinsstraat 13, 2000, Antwerp, Belgium. Electronic address: peter.goos@kuleuven.be. ^[3] imec, Kapeldreef 75, 3001, Leuven, Belgium. Electronic address: tim.stakenborg@imec.be. ^[4] Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200d, 3001, Leuven, Belgium; imec, Kapeldreef 75, 3001, Leuven, Belgium. Electronic address: liesbet.lagae@imec.be. ^[5] imec, Kapeldreef 75, 3001, Leuven, Belgium. Electronic address: dries.braeken@imec.be. ^[6]

关键词 CMOS microelectrode arrays Design of experiments Dosage control High-definition electroporation Intracellular delivery Primary cells RNA delivery Single-cell transfection Spatially-resolved multiplexing

主题词微电极(Microelectrodes);电穿孔(Electroporation);转染(Transfection);RNA, 信使(RNA, Messenger)

DOI 10.1016/j.jconrel.2022.10.001

PMID 36208793

发布时间 2024-02-21

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