Light-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencing.

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作者： Jocelyn Y,Kishi ^[1] ; Ninning,Liu ^[2] ; Emma R,West ^[3] ; Kuanwei,Sheng ^[2] ; Jack J,Jordanides ^[2] ; Matthew,Serrata ^[2] ; Constance L,Cepko ^[4] ; Sinem K,Saka ^[5] ; Peng,Yin ^[6]

作者单位： Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA. jocelyn.y.kishi@gmail.com.;Department of Systems Biology, Harvard Medical School, Boston, MA, USA. jocelyn.y.kishi@gmail.com. ^[1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA. ^[2] Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.;Howard Hughes Medical Institute, Chevy Chase, MD, USA. ^[3] Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA. cepko@genetics.med.harvard.edu.;Howard Hughes Medical Institute, Chevy Chase, MD, USA. cepko@genetics.med.harvard.edu.;Department of Ophthalmology, Harvard Medical School, Boston, MA, USA. cepko@genetics.med.harvard.edu. ^[4] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA. sinem.saka@embl.de.;Department of Systems Biology, Harvard Medical School, Boston, MA, USA. sinem.saka@embl.de.;Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. sinem.saka@embl.de. ^[5] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA. py@hms.harvard.edu.;Department of Systems Biology, Harvard Medical School, Boston, MA, USA. py@hms.harvard.edu. ^[6]

主题词动物(Animals);小鼠(Mice);高通量核苷酸序列分析(High-Throughput Nucleotide Sequencing);DNA, 互补(DNA, Complementary);DNA(DNA)

DOI 10.1038/s41592-022-01604-1

PMID 36216958

发布时间 2024-09-07

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