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Imaging mass spectrometry reveals complex lipid distributions across <i>Staphylococcus aureus</i> biofilm layers.

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第一作者： Emilio S,Rivera

第一单位： Department of Biochemistry, Vanderbilt University, Nashville, TN, USA.;Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.

作者： Emilio S,Rivera ^[1] ; Andy,Weiss ^[2] ; Lukasz G,Migas ^[3] ; Jeffrey A,Freiberg ^[2] ; Katerina V,Djambazova ^[4] ; Elizabeth K,Neumann ^[1] ; Raf,Van de Plas ^[5] ; Jeffrey M,Spraggins ^[6] ; Eric P,Skaar ^[2] ; Richard M,Caprioli ^[7]

作者单位： Department of Biochemistry, Vanderbilt University, Nashville, TN, USA.;Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA. ^[1] Department of Pathology Microbiology and Immunology, Vanderbilt University, Nashville, TN, USA. ^[2] Delft University of Technology, Delft, Netherlands. ^[3] Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.;Department of Chemistry, Vanderbilt University, Nashville, TN, USA. ^[4] Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.;Delft University of Technology, Delft, Netherlands. ^[5] Department of Biochemistry, Vanderbilt University, Nashville, TN, USA.;Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.;Department of Chemistry, Vanderbilt University, Nashville, TN, USA.;Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA. ^[6] Department of Biochemistry, Vanderbilt University, Nashville, TN, USA.;Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.;Department of Chemistry, Vanderbilt University, Nashville, TN, USA.;Department of Medicine, Vanderbilt University, Nashville, TN, USA.;Department of Pharmacology, Vanderbilt University, Nashville, TN, USA. ^[7]

关键词 Biofilms CASI, continuous accumulation of selected ions CFU, colony forming units CL, Cardiolipin CMC, carboxymethylcellulose DAN, 1′,5′-Diaminonaphthalene DAPI, 4′,6-diamidino-2-phenylindole DG, diacylglycerol DGDG, digalactosyldiacylglycerol DHA, 2′,5′-Dihydroxyacetophenone DsRed, red fluorescent protein from Discosoma EFG, electric field gradient FWHM, full-width half max GFP, green fluorescent protein IMS, imaging mass spectrometry ITO, indium-tin oxide Imaging Mass Spectrometry L-PG, lysyl-phosphatidylglycerol Lipids MALDI, matrix-assisted laser desorption/ionization OCT, optimal cutting temperature PC, phosphatidylcholine PG, phosphatidylglycerol PIP, phosphatidylinositol phosphate S/N, Signal-to-noise TIC, total ion current TIMS, trapped ion mobility spectrometry TSA, tryptic soy agar TSB, tryptic soy broth Trapped Ion Mobility XIM, extracted ion mobilogram

DOI 10.1016/j.jmsacl.2022.09.003

PMID 36388058

发布时间 2023-05-26

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Journal of mass spectrometry and advances in the clinical lab

2022年26卷

36-46页

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Journal of mass spectrometry and advances in the clinical lab

2022年26卷

36-46页

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Imaging mass spectrometry reveals complex lipid distributions across <i>Staphylococcus aureus</i> biofilm layers.

Journal of mass spectrometry and advances in the clinical lab

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Imaging mass spectrometry reveals complex lipid distributions across <i>Staphylococcus aureus</i> biofilm layers.

Journal of mass spectrometry and advances in the clinical lab

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Journal of mass spectrometry and advances in the clinical lab

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