Affinity-based and in a single step purification of recombinant horseradish peroxidase A2A isoenzyme produced by Pichia pastoris.

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作者： Melek,Acar ^[1] ; Nurgul,Abul ^[2] ; Seyda,Yildiz ^[1] ; Ezgi Dag,Taskesenligil ^[1] ; Serpil,Gerni ^[2] ; Yagmur,Unver ^[3] ; Ramazan,Kalin ^[4] ; Hasan,Ozdemir ^[5]

作者单位： Department of Molecular Biology and Genetics, Faculty of Science, Atatürk University, Erzurum, Turkey. ^[1] Department of Chemistry, Faculty of Science, Atatürk University, Erzurum, Turkey. ^[2] Department of Molecular Biology and Genetics, Faculty of Science, Atatürk University, Erzurum, Turkey. yagmurunver@yahoo.com. ^[3] Department of Basic Sciences, Faculty of Science, Erzurum Technical University, Erzurum, Turkey. ^[4] Department of Chemistry, Faculty of Science, Atatürk University, Erzurum, Turkey. hozdemir@atauni.edu.tr. ^[5]

关键词 Affinity column Extracellular expression HRP A2A isoenzyme Pichia pastoris Purification

主题词重组蛋白质类(Recombinant Proteins);同工酶类(Isoenzymes);辣根过氧化物酶(Horseradish Peroxidase);大肠杆菌(Escherichia coli);毕赤酵母(Pichia)

DOI 10.1007/s00449-022-02837-2

PMID 36527454

发布时间 2023-03-07

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