High yield expression and purification of full-length Neurotensin with pyroglutamate modification.

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第一作者： Kazem,Asadollahi

第一单位： Department of Biochemistry and Pharmacology, University of Melbourne, Parkville, VIC, 3052, Australia; Bio21 Molecular Sciences and Biotechnology Institute, University of Melbourne, Parkville, VIC, 3052, Australia; The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC, 3052, Australia.

作者： Kazem,Asadollahi ^[1] ; Katherine,Huang ^[2] ; Fei,Yan ^[2] ; Lazarus A,de Zhang ^[3] ; Daniel J,Scott ^[4] ; Paul R,Gooley ^[5]

作者单位： Department of Biochemistry and Pharmacology, University of Melbourne, Parkville, VIC, 3052, Australia; Bio21 Molecular Sciences and Biotechnology Institute, University of Melbourne, Parkville, VIC, 3052, Australia; The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC, 3052, Australia. ^[1] Department of Biochemistry and Pharmacology, University of Melbourne, Parkville, VIC, 3052, Australia; Bio21 Molecular Sciences and Biotechnology Institute, University of Melbourne, Parkville, VIC, 3052, Australia. ^[2] Department of Biochemistry and Pharmacology, University of Melbourne, Parkville, VIC, 3052, Australia; The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC, 3052, Australia; Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC, 3052, Australia. ^[3] Department of Biochemistry and Pharmacology, University of Melbourne, Parkville, VIC, 3052, Australia; The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC, 3052, Australia. ^[4] Department of Biochemistry and Pharmacology, University of Melbourne, Parkville, VIC, 3052, Australia; Bio21 Molecular Sciences and Biotechnology Institute, University of Melbourne, Parkville, VIC, 3052, Australia. Electronic address: prg@unimelb.edu.au. ^[5]

关键词 Isotope labeling Neurotensin Pyroglutamate SUMO

主题词动物(Animals);神经降压肽(Neurotensin);吡咯烷酮羧酸(Pyrrolidonecarboxylic Acid);肽类(Peptides);谷氨酰胺(Glutamine);哺乳动物(Mammals)

DOI 10.1016/j.pep.2022.106227

PMID 36574939

发布时间 2023-02-01

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Protein expression and purification

2023年204卷

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High yield expression and purification of full-length Neurotensin with pyroglutamate modification.

Protein expression and purification

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High yield expression and purification of full-length Neurotensin with pyroglutamate modification.

Protein expression and purification

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Protein expression and purification

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