Fluorescently labeled human apurinic/apyrimidinic endonuclease APE1 reveals effects of DNA polymerase β on the APE1-DNA interaction.

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作者： Artemiy S,Bakman ^[1] ; Aleksandra A,Kuznetsova ^[1] ; Lyudmila V,Yanshole ^[2] ; Alexander A,Ishchenko ^[3] ; Murat,Saparbaev ^[4] ; Olga S,Fedorova ^[1] ; Nikita A,Kuznetsov ^[5]

作者单位： Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences (SB RAS), 8 Prospekt Akad. Lavrentyeva, Novosibirsk 630090, Russia. ^[1] International Tomography Center SB RAS, 3a Institutskaya Str., Novosibirsk 630090, Russia. ^[2] Group "Mechanisms of DNA Repair and Carcinogenesis", Gustave Roussy Cancer Campus, CNRS UMR9019, Université Paris-Saclay, 94805 Villejuif, France. ^[3] Group "Mechanisms of DNA Repair and Carcinogenesis", Gustave Roussy Cancer Campus, CNRS UMR9019, Université Paris-Saclay, 94805 Villejuif, France; NCJSC "Al-Farabi Kazakh National University" Almaty, Kazakhstan. ^[4] Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences (SB RAS), 8 Prospekt Akad. Lavrentyeva, Novosibirsk 630090, Russia; Department of Natural Sciences, Novosibirsk State University, 2 Pirogova Str., Novosibirsk 630090, Russia. Electronic address: nikita.kuznetsov@niboch.nsc.ru. ^[5]

关键词 Apurinic/apyrimidinic endonuclease Conformational change DNA repair DNA‐protein interaction Damaged DNA transfer Fluorescence Pre‐steady‐state kinetics Protein-protein interaction

主题词人类(Humans);DNA(DNA);DNA损伤(DNA Damage);DNA聚合酶β(DNA Polymerase beta);DNA修复(DNA Repair);DNA-(无嘌呤或无嘧啶位点)裂合酶(DNA-(Apurinic or Apyrimidinic Site) Lyase);内切核酸酶类(Endonucleases)

DOI 10.1016/j.dnarep.2023.103450

PMID 36689867

发布时间 2023-03-17

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