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Protocol for bulk RNA sequencing of enriched human neutrophils from whole blood and estimation of sample purity.

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第一作者： Anna L K,Gonye

第一单位： Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Cellular and Molecular Medicine Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: agonye1@jhmi.edu.

作者： Anna L K,Gonye ^[1] ; Thomas J,LaSalle ^[2] ; Samuel S,Freeman ^[3] ; Miguel,Reyes ^[4] ; Nir,Hacohen ^[3] ; Alexandra-Chloé,Villani ^[5] ; Moshe,Sade-Feldman ^[6]

作者单位： Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Cellular and Molecular Medicine Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: agonye1@jhmi.edu. ^[1] Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Program in Health Sciences and Technology, Harvard Medical School & Massachusetts Institute of Technology, Boston, MA, USA. ^[2] Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA. ^[3] Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. ^[4] Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Center for Immunology and Inflammatory Diseases, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Department of Medicine, Harvard Medical School, Boston, MA, USA. ^[5] Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Harvard Medical School, Boston, MA, USA. Electronic address: msade-feldman@mgh.harvard.edu. ^[6]

关键词 Bioinformatics Cell Biology Cell Isolation Immunology Molecular Biology RNAseq Sequence Analysis Single Cell

医学主题词人类(Humans);中性白细胞(Neutrophils);白细胞(Leukocytes);脓毒症(Sepsis);碱基序列(Base Sequence);序列分析, RNA(Sequence Analysis, RNA)

DOI 10.1016/j.xpro.2023.102125

PMID 36853705

发布时间 2023-11-16

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