A review of the approaches used to solve sub-100 kDa membrane proteins by cryo-electron microscopy.

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第一作者： Peter J,Harrison

第一单位： Membrane Protein Laboratory, Diamond Light Source, Research Complex at Harwell, Didcot, UK; Electron Bio-Imaging Centre, Diamond Light Source, Didcot, UK.

作者： Peter J,Harrison ^[1] ; Tereza,Vecerkova ^[2] ; Daniel K,Clare ^[3] ; Andrew,Quigley ^[4]

作者单位： Membrane Protein Laboratory, Diamond Light Source, Research Complex at Harwell, Didcot, UK; Electron Bio-Imaging Centre, Diamond Light Source, Didcot, UK. ^[1] Membrane Protein Laboratory, Diamond Light Source, Research Complex at Harwell, Didcot, UK; Electron Bio-Imaging Centre, Diamond Light Source, Didcot, UK; School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, UK. ^[2] Electron Bio-Imaging Centre, Diamond Light Source, Didcot, UK. Electronic address: daniel.clare@diamond.ac.uk. ^[3] Membrane Protein Laboratory, Diamond Light Source, Research Complex at Harwell, Didcot, UK. Electronic address: andrew.quigley@diamond.ac.uk. ^[4]

关键词 Atomic resolution Membrane protein Protein purification Structural biology cryo-EM

主题词膜蛋白质类(Membrane Proteins);冷冻电子显微镜检查(Cryoelectron Microscopy);分子构象(Molecular Conformation);细胞膜(Cell Membrane);标本制备(Specimen Handling)

DOI 10.1016/j.jsb.2023.107959

PMID 37004781

发布时间 2023-05-31

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