Construction of high coverage whole-genome sequencing libraries from single colon crypts without DNA extraction or whole-genome amplification.

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作者： Zarko,Manojlovic ^[1] ; Jordan,Wlodarczyk ^[2] ; Cindy,Okitsu ^[3] ; Yuxin,Jin ^[4] ; David,Van Den Berg ^[5] ; Michael R,Lieber ^[3] ; Chih-Lin,Hsieh ^[6]

作者单位： Department of Urology, University of Southern California, 1441 Eastlake Ave., NTT5420, Los Angeles, CA, USA. ^[1] Department of Surgery, University of Southern California, Los Angeles, CA, USA. ^[2] Department of Pathology, University of Southern California, Los Angeles, CA, USA. ^[3] Department of Translational Genomics, Keck School of Medicine of USC, Los Angeles, CA, USA. ^[4] Department of Population and Public Health Sciences, University of Southern California, Los Angeles, CA, USA. ^[5] Department of Urology, University of Southern California, 1441 Eastlake Ave., NTT5420, Los Angeles, CA, USA. hsieh_c@med.usc.edu. ^[6]

关键词 High coverage whole-genome sequencing Next generation sequencing Sequencing library construction without DNA extraction

主题词人类(Humans);DNA(DNA);序列分析, DNA(Sequence Analysis, DNA);聚合酶链反应(Polymerase Chain Reaction);核酸扩增技术(Nucleic Acid Amplification Techniques);高通量核苷酸序列分析(High-Throughput Nucleotide Sequencing)

DOI 10.1186/s13104-023-06333-y

PMID 37106434

发布时间 2023-06-10

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准确一般字义偏差语意偏离译文作用小提交