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Evaluation of the effect of outer primer structure, and inner primer linker sequences, in the performance of Loop-mediated isothermal amplification.

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第一作者： Alexandre,Lamas

第一单位： Food Hygiene, Inspection and Control Laboratory, Department of Analytical Chemistry, Nutrition and Bromatology, Universidad de Santiago de Compostela, 27002, Lugo, Spain.

作者： Alexandre,Lamas ^[1] ; Sarah,Azinheiro ^[2] ; Foteini,Roumani ^[2] ; Marta,Prado ^[3] ; Alejandro,Garrido-Maestu ^[4]

作者单位： Food Hygiene, Inspection and Control Laboratory, Department of Analytical Chemistry, Nutrition and Bromatology, Universidad de Santiago de Compostela, 27002, Lugo, Spain. ^[1] International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga s/n, 4715-330, Braga, Portugal; Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Veterinary Science, University of Santiago de Compostela, 27002, Lugo, Spain. ^[2] International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga s/n, 4715-330, Braga, Portugal. ^[3] International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga s/n, 4715-330, Braga, Portugal. Electronic address: alejandro.garrido@inl.int. ^[4]

关键词 DPO Dual-priming oligonucleotide L. monocytogenes Linker Loop-mediated isothermal amplification

医学主题词敏感性与特异性(Sensitivity and Specificity);核酸扩增技术(Nucleic Acid Amplification Techniques);寡核苷酸类(Oligonucleotides);分子诊断技术(Molecular Diagnostic Techniques);DNA, 细菌(DNA, Bacterial);DNA引物(DNA Primers)

DOI 10.1016/j.talanta.2023.124642

PMID 37167680

发布时间 2023-05-29

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准确一般字义偏差语意偏离译文作用小提交