Preparation of hyaluronan oligosaccharides by a prokaryotic beta-glucuronidase: Characterization of free and immobilized forms of the enzyme.

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作者： Sofia,Chatzigeorgiou ^[1] ; Jana,Jílková ^[2] ; Lucie,Korecká ^[3] ; Radka,Janyšková ^[4] ; Martina,Hermannová ^[4] ; Matej,Šimek ^[4] ; Dagmar,Čožíková ^[4] ; Marcela,Slováková ^[5] ; Zuzana,Bílková ^[5] ; Jan,Bobek ^[6] ; Zbyněk,Černý ^[4] ; Matouš,Čihák ^[1] ; Vladimír,Velebný ^[4]

作者单位： Contipro a.s., Dolní Dobrouč 401, 56102 Dolní Dobrouč, Czech Republic; Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic. ^[1] Contipro a.s., Dolní Dobrouč 401, 56102 Dolní Dobrouč, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic. Electronic address: jilkova@contipro.com. ^[2] Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic. Electronic address: lucie.korecka@upce.cz. ^[3] Contipro a.s., Dolní Dobrouč 401, 56102 Dolní Dobrouč, Czech Republic. ^[4] Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic. ^[5] Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic; Faculty of Science, Jan Evangelista Purkyně University in Ústí nad Labem, České mládeže 8, 400 96 Ústí nad Labem, Czech Republic; Faculty of Biomedical Engineering, Czech Technical University in Prague, Sítná sq. 3105, 272 01 Kladno, Czech Republic. ^[6]

关键词 Bacteroides fragilis Beta-glucuronidase Hyaluronan Immobilization Oligosaccharides

主题词葡糖醛酸糖苷酶(Glucuronidase);透明质酸(Hyaluronic Acid);酶类, 固相(Enzymes, Immobilized);寡糖类(Oligosaccharides);水解作用(Hydrolysis)

DOI 10.1016/j.carbpol.2023.121078

PMID 37364952

发布时间 2023-06-28

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