Development of a Simple Direct and Hot-Start PCR Using <i>Escherichia coli</i>-Expressing <i>Taq</i> DNA Polymerase.

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作者： Sun Ju,Lee ^[1] ; Sang-Yong,Park ^[2] ; Kwang-Ho,Lee ^[1] ; Min-Woo,Lee ^[1] ; Chae-Yeon,Yu ^[3] ; Jaeyoung,Maeng ^[1] ; Hyeong-Dong,Kim ^[1] ; Suhng Wook,Kim ^[2]

作者单位： Department of Health and Safety Convergence Science, Graduate School, Korea University, 145 Anam-ro, Seoul 02841, Republic of Korea. ^[1] L-HOPE Program for Community-Based Total Learning Health Systems, Korea University, 145 Anam-ro, Seoul 02841, Republic of Korea. ^[2] Department of Laboratory Medicine, ASAN Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. ^[3] Graduate School of Particulate Matter Specialization, Korea University, 145 Anam-ro, Seoul 02841, Republic of Korea. ^[4]

关键词 Taq DNA polymerase direct PCR hot-start PCR whole blood

主题词 Taq聚合酶(Taq Polymerase);大肠杆菌(Escherichia coli);聚合酶链反应(Polymerase Chain Reaction);DNA(DNA);DNA复制(DNA Replication)

DOI 10.3390/ijms241411405

PMID 37511160

发布时间 2023-07-31

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