Development of a CRISPR/Cas9<sup>D10A</sup> Nickase (nCas9)-Mediated Genome Editing Tool in <i>Streptomyces</i>.

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作者： Jia-Xiang,Ma ^[1] ; Wen-Yan,He ^[1] ; Hui-Min,Hua ^[1] ; Qian,Zhu ^[1] ; Guo-Song,Zheng ^[1] ; Andrei A,Zimin ^[2] ; Wen-Fang,Wang ^[1] ; Yin-Hua,Lu ^[1]

作者单位： College of Life Sciences, Shanghai Normal University, Shanghai 200234, China. ^[1] G.K. Scriabin Institute of Biochemistry and Physiology of Microorganisms RAS, Pushchino 142290, Russia. ^[2] Shanghai Collaborative Innovation Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China. ^[3]

关键词 CRISPR/Cas9 Streptomyces genome editing low-toxicity nickase

主题词人类(Humans);脱氧核糖核酸酶Ⅰ(Deoxyribonuclease I);链霉菌属(Streptomyces);DNA(DNA);放线菌目(Actinomycetales)

DOI 10.1021/acssynbio.3c00466

PMID 37722085

发布时间 2023-10-24

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准确一般字义偏差语意偏离译文作用小提交