Enhanced expression of a novel trypsin from Streptomyces fradiae in Komagataella phaffii GS115 through combinational strategies of propeptide engineering and self-degredation sites modification.

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第一作者： Chengtuo,Niu

第一单位： Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Lab of Brewing Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.

作者： Chengtuo,Niu ^[1] ; Guozheng,Liu ^[2] ; Shijing,Yang ^[1] ; Linman,He ^[1] ; Chunfeng,Liu ^[1] ; Feiyun,Zheng ^[1] ; Jinjing,Wang ^[1] ; Qi,Li ^[3]

作者单位： Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Lab of Brewing Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China. ^[1] School of Biotechnology, Jiangnan University, Wuxi 214122, China. ^[2] Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Lab of Brewing Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: liqi@jiangnan.edu.cn. ^[3]

关键词 Expression level Fermentation optimization Propeptide Self-degradation Trypsin

主题词动物(Animals);牛(Cattle);毕赤酵母(Pichia);胰蛋白酶(Trypsin);酵母菌目(Saccharomycetales);青霉素类(Penicillins);抗感染药(Anti-Infective Agents)

DOI 10.1016/j.ijbiomac.2023.127382

PMID 37838138

发布时间 2024-01-06

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