Mass spectrometric assays monitoring the deubiquitinase activity of the SARS-CoV-2 papain-like protease inform on the basis of substrate selectivity and have utility for substrate identification.

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作者： Lennart,Brewitz ^[1] ; H T,Henry Chan ^[2] ; Petra,Lukacik ^[3] ; Claire,Strain-Damerell ^[3] ; Martin A,Walsh ^[3] ; Fernanda,Duarte ^[2] ; Christopher J,Schofield ^[4]

作者单位： Chemistry Research Laboratory, Department of Chemistry, University of Oxford, 12 Mansfield Road, OX1 3TA Oxford, United Kingdom; The Ineos Oxford Institute for Antimicrobial Research, Department of Chemistry, University of Oxford, 12 Mansfield Road, OX1 3TA Oxford, United Kingdom. Electronic address: lennart.brewitz@chem.ox.ac.uk. ^[1] Chemistry Research Laboratory, Department of Chemistry, University of Oxford, 12 Mansfield Road, OX1 3TA Oxford, United Kingdom. ^[2] Diamond Light Source Ltd., Harwell Science and Innovation Campus, OX11 0DE Didcot, United Kingdom; Research Complex at Harwell, Harwell Science and Innovation Campus, OX11 0FA Didcot, United Kingdom. ^[3] Chemistry Research Laboratory, Department of Chemistry, University of Oxford, 12 Mansfield Road, OX1 3TA Oxford, United Kingdom; The Ineos Oxford Institute for Antimicrobial Research, Department of Chemistry, University of Oxford, 12 Mansfield Road, OX1 3TA Oxford, United Kingdom. Electronic address: christopher.schofield@chem.ox.ac.uk. ^[4]

关键词 Deubiquitinase / DUB Interferon regulatory factor 3 / IRF3 Interferon stimulated gene 15 / ISG15 Mass spectrometry Nucleophilic cysteine protease SARS-CoV-2 papain-like protease / PL(pro)Ubiquitin Ubiquitin-like modifier Virus-host interactions eIF4E-homologous protein / 4EHP

主题词人类(Humans);赖氨酸(Lysine);病毒蛋白质类(Viral Proteins);泛素(Ubiquitin);寡肽类(Oligopeptides)

DOI 10.1016/j.bmc.2023.117498

PMID 37857256

发布时间 2024-03-16

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