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Dual spatially resolved transcriptomics for human host-pathogen colocalization studies in FFPE tissue sections.

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第一作者： Hailey,Sounart

第一单位： Department of Gene Technology, KTH Royal Institute of Technology, SciLifeLab, Stockholm, Sweden.

作者： Hailey,Sounart ^[1] ; Enikő,Lázár ^[2] ; Yuvarani,Masarapu ^[1] ; Jian,Wu ^[1] ; Tibor,Várkonyi ^[3] ; Tibor,Glasz ^[3] ; András,Kiss ^[3] ; Erik,Borgström ^[4] ; Andrew,Hill ^[4] ; Sefanit,Rezene ^[5] ; Soham,Gupta ^[5] ; Aleksandra,Jurek ^[4] ; Anezka,Niesnerová ^[4] ; Henrik,Druid ^[6] ; Olaf,Bergmann ^[7] ; Stefania,Giacomello ^[8]

作者单位： Department of Gene Technology, KTH Royal Institute of Technology, SciLifeLab, Stockholm, Sweden. ^[1] Department of Gene Technology, KTH Royal Institute of Technology, SciLifeLab, Stockholm, Sweden.;Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden. ^[2] 2nd Department of Pathology, Semmelweis University, Budapest, Hungary. ^[3] 10X Genomics, Pleasanton, CA, USA. ^[4] Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden. ^[5] Department of Oncology-Pathology, Karolinska Institutet, 17177, Stockholm, Sweden. ^[6] Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.;Center for Regenerative Therapies Dresden (CRTD), TU Dresden, Dresden, Germany.;Universitätsmedizin Göttingen, Institute of Pharmacology and Toxicology, Göttingen, Germany. ^[7] Department of Gene Technology, KTH Royal Institute of Technology, SciLifeLab, Stockholm, Sweden. stefania.giacomello@scilifelab.se. ^[8]

关键词 Colocalization analysis Formalin-fixed paraffin-embedded (FFPE) tissues Host–pathogen interactions Spatial transcriptomics

医学主题词人类(Humans);组织固定(Tissue Fixation);甲醛(Formaldehyde)

DOI 10.1186/s13059-023-03080-y

PMID 37858234

发布时间 2024-10-13

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