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Effector Translocation Assay: Differential Solubilization.

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第一作者： Irina S,Franco

第一单位： Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, Caparica, Portugal.;UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, NOVA University Lisbon, Caparica, Portugal.

作者： Irina S,Franco ^[1] ; Sara V,Pais ^[2] ; Nuno,Charro ^[2] ; Luís Jaime,Mota ^[3]

作者单位： Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, Caparica, Portugal.;UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, NOVA University Lisbon, Caparica, Portugal. ^[1] UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, NOVA University Lisbon, Caparica, Portugal. ^[2] Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, Caparica, Portugal. ljmota@fct.unl.pt.;UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, NOVA University Lisbon, Caparica, Portugal. ljmota@fct.unl.pt. ^[3]

关键词 Bacterial protein secretion system Detergent solubilization Effector; translocation Immunoblotting SDS-PAGE Type III secretion