Handling Difficult Cryo-ET Samples: A Study with Primary Neurons from Drosophila melanogaster.

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第一作者： Joseph Y,Kim

第一单位： Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.

作者： Joseph Y,Kim ^[1] ; Jie E,Yang ^[2] ; Josephine W,Mitchell ^[1] ; Lauren A,English ^[3] ; Sihui Z,Yang ^[4] ; Tanner,Tenpas ^[5] ; Erik W,Dent ^[6] ; Jill,Wildonger ^[7] ; Elizabeth R,Wright ^[8]

作者单位： Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA. ^[1] Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA. ^[2] Cryo-Electron Microscopy Research Center, University of Wisconsin-Madison, Madison, WI 53706, USA. ^[3] Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA. ^[4] Department of Chemistry and Biochemistry, Kalamazoo College, Kalamazoo, MI 49006, USA. ^[5] Neuroscience Training Program, University of Wisconsin-Madison, Madison, WI 53705, USA. ^[6] Department of Biology, Stanford University, Stanford, CA 94305, USA. ^[7] Department of Neuroscience, University of Wisconsin-Madison, Madison, WI 53705, USA. ^[8] Departments of Pediatrics and Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA. ^[9] Morgridge Institute for Research, University of Wisconsin-Madison, Madison, WI 53715, USA. ^[10]

关键词 Drosophila melanogaster chemical fixation cryo-electron microscopy (cryo-EM)cryo-electron tomography (cryo-ET)fluorescence light microscopy membrane blebs micropatterning primary neurons varicosity

主题词动物(Animals);黑腹果蝇(Drosophila melanogaster);神经元(Neurons);电子显微镜体层摄影术(Electron Microscope Tomography);冷冻电子显微镜检查(Cryoelectron Microscopy)

DOI 10.1093/micmic/ozad125

PMID 37966978

发布时间 2024-06-13

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