Sequence- and Structure-Based Mining of Thermostable D-Allulose 3-Epimerase and Computer-Guided Protein Engineering To Improve Enzyme Activity.

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第一作者： Hongbin,Qi

第一单位： Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, National Engineering Laboratory for Industrial Enzymes, Tianjin 300457, China.

作者： Hongbin,Qi ^[1] ; Tong,Wang ^[1] ; Huimin,Li ^[1] ; Chao,Li ^[1] ; Lijun,Guan ^[2] ; Weidong,Liu ^[3] ; Jianwen,Wang ^[4] ; Fuping,Lu ^[1] ; Shuhong,Mao ^[1] ; Hui-Min,Qin ^[1]

作者单位： Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, National Engineering Laboratory for Industrial Enzymes, Tianjin 300457, China. ^[1] Institute of Food Processing, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China. ^[2] Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. ^[3] Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. ^[4]

关键词 D-allulose 3-epimerase big data mining in silico screening semirational design thermostability

主题词消旋酶类和差向异构酶类(Racemases and Epimerases);果糖(Fructose);蛋白质工程(Protein Engineering);甜味剂(Sweetening Agents);酶稳定性(Enzyme Stability)

DOI 10.1021/acs.jafc.3c07204

PMID 37970673

发布时间 2023-11-30

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