Protocatechuate hydroxylase is a novel group A flavoprotein monooxygenase with a unique substrate recognition mechanism.

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作者： Nozomi,Katsuki ^[1] ; Riku,Fukushima ^[2] ; Yuki,Doi ^[1] ; Shunsuke,Masuo ^[1] ; Takatoshi,Arakawa ^[3] ; Chihaya,Yamada ^[4] ; Shinya,Fushinobu ^[5] ; Naoki,Takaya ^[6]

作者单位： Faculty of Life and Environmental Sciences, Microbiology Research Center for Sustainability, University of Tsukuba, Tsukuba, Ibaraki, Japan. ^[1] Department of Biotechnology, The University of Tokyo, Tokyo, Japan. ^[2] Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan. ^[3] School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan. ^[4] Department of Biotechnology, The University of Tokyo, Tokyo, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan. Electronic address: asfushi@mail.ecc.u-tokyo.ac.jp. ^[5] Faculty of Life and Environmental Sciences, Microbiology Research Center for Sustainability, University of Tsukuba, Tsukuba, Ibaraki, Japan. Electronic address: takaya.naoki.ge@u.tsukuba.ac.jp. ^[6]

关键词 crystal structure enzyme kinetics flavin adenine dinucleotide p-hydroxybenzoate hydroxylase pre-steady state kinetics pyridine nucleotide reductive half-reaction

主题词 4-羟基苯甲酸盐-3-单氧化酶(4-Hydroxybenzoate-3-Monooxygenase);结合部位(Binding Sites);黄素蛋白类(Flavoproteins);动力学(Kinetics);混合功能氧化酶类(Mixed Function Oxygenases);种系发生(Phylogeny);假单胞菌属(Pseudomonas)

DOI 10.1016/j.jbc.2023.105508

PMID 38029967

发布时间 2025-02-10

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准确一般字义偏差语意偏离译文作用小提交