Quencher-free fluorescent assays by controlled DNA partitioning in the aqueous two-phase system with crowding-enhanced kinetics.

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作者： Haoyue,Lv ^[1] ; Xiaoman,Duan ^[1] ; Zhaoyu,Han ^[1] ; Haozhen,Yu ^[1] ; Biwu,Liu ^[2]

作者单位： Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, PR China. ^[1] Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, PR China. Electronic address: biwuliu@xjtu.edu.cn. ^[2]

关键词 Excluded volume Fluorescent assays Phase separation Quencher-free Strand displacement

主题词生物传感技术(Biosensing Techniques);DNA(DNA);微RNAs(MicroRNAs);荧光染料(Fluorescent Dyes);核酸扩增技术(Nucleic Acid Amplification Techniques)

DOI 10.1016/j.bios.2023.115864

PMID 38039730

发布时间 2024-01-01

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准确一般字义偏差语意偏离译文作用小提交