Expression and Purification of Recombinant Human Mitochondrial RNA Polymerase (POLRMT) and the Initiation Factors TFAM and TFB2M.

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作者： An H,Hsieh ^[1] ; Sean D,Reardon ^[1] ; Jubilee H,Munozvilla-Cabellon ^[1] ; Jiayu,Shen ^[2] ; Smita S,Patel ^[2] ; Tatiana V,Mishanina ^[1]

作者单位： Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA. ^[1] Department of Biochemistry and Molecular Biology, Rutgers University, Robert Wood Johnson Medical School, Piscataway, NJ, USA. ^[2]

关键词 Bacterial protein expression Heparin Maltose binding protein (MBP) fusion protein purification Ni-NTA POLRMT Protein purification TEV protease TFAM TFB2M

DOI 10.21769/BioProtoc.4892

PMID 38094251

发布时间 2024-01-31

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Bio-protocol

2023年13卷23期

e4892页

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Expression and Purification of Recombinant Human Mitochondrial RNA Polymerase (POLRMT) and the Initiation Factors TFAM and TFB2M.

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Expression and Purification of Recombinant Human Mitochondrial RNA Polymerase (POLRMT) and the Initiation Factors TFAM and TFB2M.

Bio-protocol

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Bio-protocol

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