Engineering the cofactor binding site of 7α-hydroxysteroid dehydrogenase for improvement of catalytic activity, thermostability, and alteration of substrate preference.

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作者： Yinping,Pan ^[1] ; Liancai,Zhu ^[2] ; Jun,Tan ^[3] ; Deshuai,Lou ^[3] ; Bochu,Wang ^[4]

作者单位： Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing 400045, PR China. ^[1] Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing 400045, PR China. Electronic address: zhuliancai75@126.com. ^[2] Chongqing Key Laboratory of Medicinal Resources in the Three Gorges Reservoir Region, School of Biological & Chemical Engineering, Chongqing University of Education, Chongqing 400067, PR China. ^[3] Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing 400045, PR China. Electronic address: wangbc2000@126.com. ^[4]

关键词 Activity-thermostability enhancement Cofactor binding site Hydroxysteroid dehydrogenase Molecular structure analysis Substrate preference

主题词细菌(Bacteria);羟甾类脱氢酶类(Hydroxysteroid Dehydrogenases);胆汁酸类和盐类(Bile Acids and Salts);结合部位(Binding Sites);动力学(Kinetics)

DOI 10.1016/j.ijbiomac.2023.128847

PMID 38123031

发布时间 2024-02-08

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