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Dissociation of murine oral mucosal tissues for single cell applications.

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第一作者： Tomoko,Ikeuchi

第一单位： Oral Immunity and Infection Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: tomoko.ikeuchi@nih.gov.

作者： Tomoko,Ikeuchi ^[1] ; Ramin,Akhi ^[2] ; Belmaliz,Cardona Rodriguez ^[2] ; David,Fraser ^[2] ; Drake,Williams ^[2] ; Tae Sung,Kim ^[2] ; Teresa,Greenwell-Wild ^[2] ; Andrew,Overmiller ^[3] ; Maria,Morasso ^[3] ; Niki,Moutsopoulos ^[4]

作者单位： Oral Immunity and Infection Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: tomoko.ikeuchi@nih.gov. ^[1] Oral Immunity and Infection Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. ^[2] Laboratory of Skin Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH, Bethesda, MD 20892, USA. ^[3] Oral Immunity and Infection Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: nmoutsopoulos@dir.nidr.nih.gov. ^[4]

关键词 Enzymatic tissue dissociation method Inflammation Oral mucosa Recovery of immune and non-immune types Single cell

医学主题词动物(Animals);小鼠(Mice);口腔黏膜(Mouth Mucosa);流式细胞术(Flow Cytometry);胶原酶类(Collagenases);炎症(Inflammation);牙周炎(Periodontitis)

DOI 10.1016/j.jim.2023.113605

PMID 38142927

发布时间 2025-05-30

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