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Human organotypic brain slice cultures: a detailed and improved protocol for preparation and long-term maintenance.

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第一作者： Aniella,Bak

第一单位： Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany. Electronic address: aniella.va.bak@gmail.com.

作者： Aniella,Bak ^[1] ; Henner,Koch ^[2] ; Karen M J,van Loo ^[3] ; Katharina,Schmied ^[2] ; Birgit,Gittel ^[2] ; Yvonne,Weber ^[2] ; Jonas,Ort ^[4] ; Niklas,Schwarz ^[5] ; Simone C,Tauber ^[6] ; Thomas V,Wuttke ^[7] ; Daniel,Delev ^[8]

作者单位： Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany. Electronic address: aniella.va.bak@gmail.com. ^[1] Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany. ^[2] Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany; Department of Neurosurgery, RWTH Aachen University, Aachen, Germany. ^[3] Department of Neurosurgery, RWTH Aachen University, Aachen, Germany. ^[4] Department of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany. ^[5] Department of Neurology, RWTH Aachen University Hospital, Aachen, Germany. ^[6] Department of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; Department of Neurosurgery, University of Tübingen, Tübingen, Germany. ^[7] Department of Neurosurgery, RWTH Aachen University, Aachen, Germany; Neurosurgical Artificial Intelligence Laboratory Aachen (NAILA), RWTH Aachen University Hospital, Aachen, Germany; Department of Neurosurgery, University of Erlangen, Erlangen, Germany. ^[8]

关键词 Human brain Human cerebrospinal fluid In vitro models Multi-electrode array Neuronal network Organotypic brain slice cultures Single-cell patch-clamp recordings Viral transduction

医学主题词人类(Humans);脑(Brain);神经元(Neurons);电极(Electrodes);器官培养技术(Organ Culture Techniques)

DOI 10.1016/j.jneumeth.2023.110055

PMID 38184112

发布时间 2024-03-29

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