Integration of IFAST-based nucleic acid extraction and LAMP for on-chip rapid detection of Agroathelia rolfsii in soil.

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作者： Phanupong,Changtor ^[1] ; Pablo,Rodriguez-Mateos ^[2] ; Kittisak,Buddhachat ^[3] ; Wandee,Wattanachaiyingcharoen ^[4] ; Alexander,Iles ^[2] ; Sutthichat,Kerdphon ^[5] ; Nonglak,Yimtragool ^[6] ; Nicole,Pamme ^[7]

作者单位： Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden; Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand. ^[1] Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden. ^[2] Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand. ^[3] Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand; Center of Excellence for Biodiversity, Faculty of Science, Naresuan University, Phitsanulok, Thailand. ^[4] Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok, Thailand. ^[5] Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand; Center of Excellence for Biodiversity, Faculty of Science, Naresuan University, Phitsanulok, Thailand. Electronic address: nonglakp@nu.ac.th. ^[6] Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden. Electronic address: nicole.pamme@mmk.su.se. ^[7]

关键词 Agroathelia rolfsii DNA extraction Immiscible filtration LAMP Magnetic particle

主题词核酸类(Nucleic Acids);表面张力(Surface Tension);生物传感技术(Biosensing Techniques);核酸扩增技术(Nucleic Acid Amplification Techniques);DNA(DNA);DNA引物(DNA Primers);敏感性与特异性(Sensitivity and Specificity)

DOI 10.1016/j.bios.2024.116051

PMID 38301544

发布时间 2024-02-14

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