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Scalable manufacture of therapeutic mesenchymal stromal cell products on customizable microcarriers in vertical wheel bioreactors that improve direct visualization, product harvest, and cost.

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第一作者： Andrew,Haskell

第一单位： Department of Cell Biology and Genetics, Texas A&M School of Medicine, Bryan, Texas, USA.

作者： Andrew,Haskell ^[1] ; Berkley P,White ^[2] ; Robert E,Rogers ^[1] ; Erin,Goebel ^[3] ; Megan G,Lopez ^[1] ; Andrew E,Syvyk ^[4] ; Daniela A,de Oliveira ^[5] ; Heather A,Barreda ^[1] ; Joshua,Benton ^[1] ; Oscar R,Benavides ^[2] ; Sujata,Dalal ^[1] ; EunHye,Bae ^[1] ; Yu,Zhang ^[1] ; Kristen,Maitland ^[6] ; Zivko,Nikolov ^[5] ; Fei,Liu ^[1] ; Ryang Hwa,Lee ^[1] ; Roland,Kaunas ^[7] ; Carl A,Gregory ^[8]

作者单位： Department of Cell Biology and Genetics, Texas A&M School of Medicine, Bryan, Texas, USA. ^[1] Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA. ^[2] Department of Cell Biology and Genetics, Texas A&M School of Medicine, Bryan, Texas, USA; Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA. ^[3] National Center for Therapeutics Manufacturing, Texas A&M University, College Station, Texas, USA. ^[4] National Center for Therapeutics Manufacturing, Texas A&M University, College Station, Texas, USA; Biological and Agricultural Engineering, Texas A&M University, College Station, Texas, USA. ^[5] Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA; Imaging Program, Chan Zuckerberg Initiative, Redwood City, California, USA. ^[6] Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA. Electronic address: rkaunas@tamu.edu. ^[7] Department of Cell Biology and Genetics, Texas A&M School of Medicine, Bryan, Texas, USA. Electronic address: cgregory@tamu.edu. ^[8]

关键词 bioreactor cell culture gelatin methacryloyl microcarriers mesenchymal stromal cells stem cell manufacture stem cell therapy