Cryopreservation of cerebrospinal fluid cells preserves the transcriptional landscape for single-cell analysis.

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作者： Mahesh Chandra,Kodali ^[1] ; Jerry,Antone ^[2] ; Eric,Alsop ^[2] ; Rojashree,Jayakumar ^[3] ; Khushi,Parikh ^[2] ; Aude,Chiot ^[4] ; Paula,Sanchez-Molina ^[4] ; Bahareh,Ajami ^[4] ; Steven E,Arnold ^[5] ; Kendall,Jensen ^[2] ; Sudeshna,Das ^[5] ; Marc S,Weinberg ^[6]

作者单位： Department of Neurology, Massachusetts General Hospital, Boston, MA, USA. mkodali@mgh.harvard.edu.;Harvard Medical School, Boston, MA, USA. mkodali@mgh.harvard.edu. ^[1] Neurogenomics Division, Translational Genomics Research Institute, Phoenix, AZ, USA. ^[2] Department of Neurology, Massachusetts General Hospital, Boston, MA, USA. ^[3] Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR, USA.;Department of Behavioral and Systems Neuroscience, Oregon Health and Science University, Portland, OR, USA. ^[4] Department of Neurology, Massachusetts General Hospital, Boston, MA, USA.;Harvard Medical School, Boston, MA, USA. ^[5] Department of Neurology, Massachusetts General Hospital, Boston, MA, USA. marc.weinberg@mgh.harvard.edu.;Harvard Medical School, Boston, MA, USA. marc.weinberg@mgh.harvard.edu.;Department of Psychiatry, Massachusetts General Hospital, Boston, MA, USA. marc.weinberg@mgh.harvard.edu. ^[6]

关键词 10x Alzheimer’s disease Cerebrospinal fluid Methods Neuroimmunology Omics Single-cell Transcriptomics

主题词人类(Humans);二甲亚砜(Dimethyl Sulfoxide);低温防护剂(Cryoprotective Agents);细胞, 培养的(Cells, Cultured);低温保存(Cryopreservation);单细胞分析(Single-Cell Analysis);细胞存活(Cell Survival)

DOI 10.1186/s12974-024-03047-1

PMID 38521932

发布时间 2024-09-28

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