Functional expression, purification, biochemical and biophysical characterizations, and molecular dynamics simulation of a histidine acid phosphatase from Saccharomyces cerevisiae.

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作者： Nima Ghahremani,Nezhad ^[1] ; Siti Zahra Binti,Jamaludin ^[2] ; Raja Noor Zaliha Raja Abd,Rahman ^[1] ; Normi Mohd,Yahaya ^[2] ; Siti Nurbaya,Oslan ^[1] ; Fairolniza Mohd,Shariff ^[3] ; Nurulfiza Mat,Isa ^[1] ; Thean Chor,Leow ^[2]

作者单位： Enzyme and Microbial Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, 43400 UPM, Selangor, Malaysia. ^[1] Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, 43400 UPM, Selangor, Malaysia. ^[2] Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, 43400 UPM, Selangor, Malaysia. ^[3] Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, 43400 UPM, Selangor, Malaysia. ^[4] Laboratory of Vaccine and Biomolecules (VacBio), Institute of Bioscience, Universiti Putra Malaysia, Serdang, 43400 UPM, Selangor, Malaysia. ^[5] Enzyme and Microbial Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, 43400 UPM, Selangor, Malaysia. adamleow@upm.edu.my. ^[6] Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, 43400 UPM, Selangor, Malaysia. adamleow@upm.edu.my. ^[7] Institute of Bioscience, Universiti Putra Malaysia, Serdang, 43400 UPM, Selangor, Malaysia. adamleow@upm.edu.my. ^[8]

关键词 Biophysicochemical characterization Functional expression Histidine acid phosphatase

主题词分子动力学模拟(Molecular Dynamics Simulation);酸性磷酸酶(Acid Phosphatase);酿酒酵母菌(Saccharomyces cerevisiae);组氨酸(Histidine);酿酒酵母蛋白质类(Saccharomyces cerevisiae Proteins);镁(Magnesium);放射性同位素(Radioisotopes)

DOI 10.1007/s11274-024-03970-8

PMID 38630327

发布时间 2024-05-17

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