A Novel Method for Floxed Gene Manipulation Using TAT-Cre Recombinase in Ex Vivo Precision-Cut Lung Slices (PCLS).

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作者： Sek-Shir,Cheong ^[1] ; Tiago C,Luis ^[2] ; Matthew,Hind ^[3] ; Charlotte H,Dean ^[1]

作者单位： National Heart and Lung Institute (NHLI), Imperial College London, London, UK. ^[1] Centre for Inflammatory Diseases, Department of Immunology and Inflammation, Imperial College London, London, UK. ^[2] National Heart and Lung Institute (NHLI), Imperial College London, London, UK.;National Institute for Health Research (NIHR) Respiratory Biomedical Research Unit, Royal Brompton & Harefield NHS Foundation Trust, London, UK. ^[3]

关键词 Ex vivo model Floxed allele modification Gene activation Gene deletion Gene modification PCLS Permanent gene manipulation Precision-cut lung slices TAT-Cre recombinase TReATS

DOI 10.21769/BioProtoc.4980

PMID 38686349

发布时间 2025-01-05

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Bio-protocol

2024年14卷8期

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A Novel Method for Floxed Gene Manipulation Using TAT-Cre Recombinase in Ex Vivo Precision-Cut Lung Slices (PCLS).

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A Novel Method for Floxed Gene Manipulation Using TAT-Cre Recombinase in Ex Vivo Precision-Cut Lung Slices (PCLS).

Bio-protocol

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Bio-protocol

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