Preamplification-free ultra-fast and ultra-sensitive point-of-care testing via LwaCas13a.

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作者： Wanting,Zeng ^[1] ; Wanping,Chen ^[1] ; Yang,Liu ^[1] ; Ting,Zhang ^[2] ; Chao,Zhai ^[1] ; Wenqiang,Li ^[1] ; Longyu,Wang ^[1] ; Cheng,Zhang ^[1] ; Qili,Zeng ^[2] ; Fei,Wang ^[3] ; Lixin,Ma ^[4]

作者单位： State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Province Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, China. ^[1] Hubei Provincial Center for Disease Control and Prevention, Wuhan, 430079, China. ^[2] State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Province Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, China. Electronic address: wangfei@hubu.edu.cn. ^[3] State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Province Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, China. Electronic address: malixing@hubu.edu.cn. ^[4]

关键词 CRISPR/Cas13a Lateral flow Point-of-care testing Preamplification-free SARS-CoV-2

主题词生物传感技术(Biosensing Techniques);人类(Humans);RNA, 病毒(RNA, Viral);核酸扩增技术(Nucleic Acid Amplification Techniques);床旁诊断化验信息系统(Point-of-Care Systems);检测限(Limit of Detection)

DOI 10.1016/j.bios.2024.116400

PMID 38776799

发布时间 2024-06-01

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